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In 2010, the UK government funded the restoration of organs from patients who had died of non-traumatic circulatory arrest (NCA) and organ donation after circulatory death (DoCD). Prior to this, there was a significant shortage of organs for transplantation. This permitted more patients to die in the UK than in other countries, while at the same time patients died from a lack of access to transplantable organs. The restoration of organs from NCA patients is a challenge not only to the clinical team, but also to legal, ethical, and medico-social teams. This review summarises the current status of NCA patients in the UK, including the legal framework, clinical teams, and some contemporary UK transplantation practices.Multiple serotypes of dengue (DEN) virus in a single blood sample using MALDI-TOF mass spectrometry.
Numerous outbreak of dengue fever are still reported every year by WHO. Rapid diagnosis and confirmatory test for dengue virus infection in clinical samples is crucial for both laboratory and clinical applications. In this study, we have developed a simple sample preparation technique using the matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) that can detect dengue virus subtypes in real-time after extraction of total RNA from a single blood sample. To optimise assay, fragment analysis was performed using 9V8 peptide derived from envelope protein of DEN-1, DEN-2, DEN-3, and DEN-4 dengue virus serotypes. The intensities of the MALDI-TOF MS signal at m/z 2796 (DEN-1), m/z
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